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pgcA [2018-04-11 10:42:33]
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pgcA [2018-04-11 10:42:33]

alpha-phosphoglucomutase, required for UDP-glucose synthesis, inhibits FtsZ ring assembly (indirect effect due to a defect in UDP-glucose synthesis)
Locus
BSU09310
Isoelectric point
4.91
Molecular weight
62.73 kDa
Protein length
565 aa Sequence Blast
Gene length
1695 bp Sequence Blast
Function
interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate
Product
alpha-phosphoglucomutase
Essential
no
E.C.
5.4.2.2
Synonyms
yhxB,gtaC,gtaE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of teichoic acid
  • Metabolism → Lipid metabolism → Lipid metabolism/ other
  • Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
  • Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of teichoic acid
  • Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

    • Gene

    Coordinates
    1,006,774 → 1,008,519

    Phenotypes of a mutant

  • cells are bent and distended due to the lack of glucolipids PubMed
  • the inactivation of pgcA suppresses the poor and filamentous growth of the whiA zapA double mutant PubMed
  • inactivation of pgcA reduces sporulation efficiency to 0.1% that of wild type cells; smaller and wider mother cells with small forespores PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate (according to Swiss-Prot)

    • Protein family

  • phosphohexose mutase family (according to Swiss-Prot)

    • Modification

  • phosphorylated on ser/ thr/ tyr PubMed, PubMed

    • Structure

  • 2Z0F (from Thermus thermophilus, 27% identity)

    • Additional information

  • PgcA inhibits FtsZ ring assembly (indirect effect due to a defect in UDP-glucose synthesis)PubMed

    • Expression and Regulation

    Operons

    Genes
    pgcA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-B476 (yhxB::erm), available at the NBRP B. subtilis, Japan
  • GP1743: BSB1 pgcA::aphA3, available in Jörg Stülke' lab
  • BKE09310 (ΔpgcA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG
  • BKK09310 (ΔpgcA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG

    • References

    Reviews

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    Original publications

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